Anti-Aging · Reference
Epithalon Dosage: How Pepti Prescribes It, in Units, mL and mg
This page explains the epithalon dosage Pepti prescribes, what the units on the syringe mean, a chart that converts units to millilitres and milligrams, the ETERNAL blend that also contains epithalon, how long a vial lasts, why courses are intermittent, what the research did and did not dose, and the side effects to know about. It covers how Pepti's epithalon is typically prescribed, not protocols from the internet. Nothing here is a reason to change your own dose.

At Pepti, epithalon is typically prescribed as 0.25 mL, which is 25 units on a U-100 insulin syringe, injected under the skin once daily Monday through Friday. The vial is 5 mL at 2 mg/mL, so each dose carries 0.5 mg of epithalon and the vial holds 20 doses, about four weeks. There is no human dose-finding study for injectable epithalon, so no published human dose exists to compare it with. Your physician sets your directions after reviewing your intake, and your exact dose is printed on the vial.
This page explains the epithalon dosage Pepti prescribes, what the units on the syringe mean, a chart that converts units to millilitres and milligrams, the ETERNAL blend that also contains epithalon, how long a vial lasts, why courses are intermittent, what the research did and did not dose, and the side effects to know about. It covers how Pepti's epithalon is typically prescribed, not protocols from the internet. Nothing here is a reason to change your own dose.
Epithalon Dosage
The short answer
| Product | Vial | Typical directions | Epithalon per dose | Doses per vial | Price |
|---|---|---|---|---|---|
| Epithalon | 5 mL, 2 mg/mL (10 mg per vial) | Inject 0.25 mL (25 units) subcutaneously once daily Monday through Friday | 0.5 mg | 20 | $249/mo |
| ETERNAL Blend | 5 mL, epithalon 2 mg/mL with GHK-Cu, BPC-157 and TB-500 | Inject 0.25 mL (25 units) subcutaneously once daily Monday through Friday | 0.5 mg, with the other three ingredients | 20 | $279/mo |
Every price is all-in, covering medication, physician review, refill management and shipping, with no separate membership fee. Pepti is cash-pay and does not bill insurance. Epithalon is a vial drawn with an insulin syringe; there is no Pen version. These are the typical printed directions; your own directions are the ones on your vial.
Epithalon Dosage
Epithalon dosage in units, mL and mg
Pepti's directions are written in two forms, millilitres and units, because prescriptions are written in millilitres and insulin syringes are marked in units. On a U-100 insulin syringe, 1 unit is 0.01 mL, so 100 units is 1 mL and 25 units is 0.25 mL. A unit is a volume mark on the barrel, not a measure of how strong the medication is.
The milligram figure comes from the concentration on the label. Pepti's epithalon is supplied at 2 mg/mL, so:
| What you read | Value | Where it comes from |
|---|---|---|
| Dose volume | 0.25 mL | Printed on the vial |
| On a U-100 insulin syringe | 25 units | 0.25 mL x 100 units per mL |
| Concentration | 2 mg/mL | Printed on the vial |
| Epithalon per dose | 0.5 mg | 2 mg/mL x 0.25 mL |
| Total in the vial | 10 mg in 5 mL | Printed on the vial |
| Doses per vial | 20 | 5 mL divided by 0.25 mL |
That arithmetic is a way to read a label, not a way to set a dose. A vial labelled 10 mg is not a 10 mg dose: it is twenty 0.5 mg doses. Online epithalon protocols often quote doses for powder vials that the reader mixes at home, at a concentration that depends on how much water was added; a Pepti vial arrives already in solution at a fixed concentration, so those figures do not transfer; do peptides come pre-mixed explains the difference. If your label and your syringe seem to disagree, do not dose; message your care team. How long does a peptide vial last explains every number on a vial label.
Epithalon Dosage
Epithalon dosage chart: units to mL to mg
The chart below is the conversion for a 2 mg/mL vial, drawn with a U-100 insulin syringe. It is math only. It is not a menu of doses, and the only line that applies to you is the one that matches the number printed on your vial.
| Syringe mark (U-100) | Volume | Epithalon from a 2 mg/mL vial |
|---|---|---|
| 5 units | 0.05 mL | 0.1 mg |
| 10 units | 0.1 mL | 0.2 mg |
| 15 units | 0.15 mL | 0.3 mg |
| 20 units | 0.2 mL | 0.4 mg |
| 25 units (Pepti's typical directions) | 0.25 mL | 0.5 mg |
| 30 units | 0.3 mL | 0.6 mg |
| 40 units | 0.4 mL | 0.8 mg |
| 50 units | 0.5 mL | 1 mg |
| 100 units | 1 mL | 2 mg |
The rule behind every row: units divided by 100 gives millilitres, and millilitres multiplied by 2 gives milligrams.
Epithalon in the ETERNAL blend
The ETERNAL Blend holds epithalon at the same 2 mg/mL, so the same 25 unit draw delivers the same 0.5 mg of epithalon. It also delivers the other three ingredients in fixed proportion:
| Ingredient | Concentration | Per 0.25 mL (25 unit) dose | Per vial |
|---|---|---|---|
| Epithalon | 2 mg/mL | 0.5 mg | 10 mg |
| GHK-Cu | 10 mg/mL | 2.5 mg | 50 mg |
| BPC-157 | 2 mg/mL | 0.5 mg | 10 mg |
| TB-500 | 2 mg/mL | 0.5 mg | 10 mg |
There is no way to take more epithalon and less of something else from the same vial; a different balance is a different prescription. More on the blend is in what is the ETERNAL blend.
Epithalon Dosage
How long an epithalon vial lasts
At Pepti's typical directions, one 10 mg vial lasts four weeks:
| Step | Value |
|---|---|
| Dose | 25 units, 0.25 mL, 0.5 mg |
| Doses per week | 5 (Monday through Friday) |
| Doses per vial | 20 (5 mL divided by 0.25 mL) |
| Epithalon used | 20 x 0.5 mg = 10 mg, the whole vial |
| Time per vial | 20 doses divided by 5 per week = 4 weeks |
Four weeks is why refills run every 28 days, one vial per fill. Vials are multi-dose solutions shipped ready to use, refrigerated with ice packs, with sterile syringes and alcohol prep pads. Nothing is mixed at home. Keep the vial in the fridge between doses. The FDA's July 2026 briefing noted that reconstituted epithalon is stable for only days at fridge temperature in the manufacturer's data, so the beyond-use date the pharmacy prints on the label governs; it runs from first puncture. How to store peptides covers storage.
If a vial runs short before the refill, message your care team rather than adjusting anything yourself. If you miss a dose, follow your directions and do not take two doses to make up for one.
Epithalon Dosage
When epithalon is taken
Once daily, Monday through Friday
Pepti's directions say once daily Monday through Friday, with weekends off. The weekday-only pattern echoes the intermittent courses used in the Russian research, and it lines a 20-dose vial up with a 28-day refill. Weekends off are part of the schedule, not missed doses.
Time of day
The standard label does not set a time. The animal work involved shifts in evening hormone levels, melatonin and cortisol, so time of day is a reasonable thing to ask your care team about. Follow the timing on your label if one is printed.
Under the skin
Subcutaneous means into the fat layer, not the muscle. Abdomen and thigh are the usual sites, rotated so the same spot is not used repeatedly. There is no reason to inject near any particular body part; the mechanisms described are systemic. Technique is covered in how to inject peptides safely.
Directions say What it means in practice 0.25 mL (25 units) Draw to the 25 mark on a U-100 insulin syringe Subcutaneously Into the fat layer, abdomen or thigh, rotating sites Once daily One injection a day Monday through Friday Five doses a week, weekends off 20 doses per vial About four weeks, then the next refill
Epithalon Dosage
Courses rather than a permanent schedule
The protocols from the St Petersburg institute that developed epithalon were intermittent: courses separated by months. The anchor mouse study, Anisimov and colleagues in Biogerontology in 2003, gave epithalon in brief monthly courses, not continuously. Prescribers generally follow that logic rather than treating epithalon as an indefinite daily medication.
There is also a safety reason. The FDA's July 2026 review raised a concern that continuous, long-term telomerase activation could in principle allow cells to escape senescence, and the rodent work that found fewer tumours used intermittent courses totalling a few months of exposure. How long a course runs, and whether and when it is repeated, is set by your physician after reviewing your intake; do not improvise a permanent schedule. Peptide cycling and tolerance covers how courses work across peptides.
Epithalon Dosage
What the research dosed, and why there is no human reference dose
People search for "the" epithalon dose expecting a trial figure. There is not one to give:
| What exists | What it was | Why it does not give a human dose |
|---|---|---|
| Cell studies, 2003 and 2025 | Epithalon added to cultured human cells | Concentrations in a dish, not doses in a person |
| Mouse lifespan study, Biogerontology 2003 | Brief monthly courses from three months of age, one fixed dose, female mice only | An animal dose |
| Old rhesus monkeys, 2001 | A short course that raised evening melatonin | An animal dose |
| Elderly cohorts, 2003 and 2011 | Epithalamin, the bovine pineal extract, not synthetic epithalon | A different preparation |
| Night-shift workers, Russian-language study | A sublingual spray for 20 days | A different route; a urinary melatonin metabolite was measured, not sleep |
When the FDA reviewed epithalon in July 2026, it found no human pharmacokinetic data and no study of the subcutaneous route at all. The honest reading is that the injectable dose in common use comes from clinical convention, not a dose-ranging trial. That is why your directions come from a physician who has reviewed your intake. The evidence, one claimed benefit at a time, is in epithalon benefits.
Epithalon Dosage
What changes the directions
Your physician sets your directions after reviewing your intake. Things that can lead to different directions, or a different product, include:
| What can change | How it shows up |
|---|---|
| Epithalon alone or in a blend | ETERNAL has its own label and its own ingredients |
| Your medical history | A personal or strong family history of cancer, or pregnancy or breastfeeding, change the conversation |
| Your other medications | There is no established interaction list, so the intake asks for everything you take |
| Course length | Courses are intermittent; your physician sets when one ends |
| How you respond | Side effects you report go to your physician, who may revise your directions |
When directions change, the new numbers are printed on the new label. Never carry a number over from a previous fill. Bloodwork is not required for any Pepti treatment. If your physician wants labs for your care, they'll ask through your account.
Epithalon Dosage
Side effects, in summary
The full picture is in what is epithalon.
| What is reported | What it usually means | What to do |
|---|---|---|
| Injection-site redness, mild soreness, occasional bruising | The commonest complaint | Rotate sites |
| Early drowsiness or vivid dreams | Usually settles | Report it if it persists |
| A severe or spreading reaction, facial or throat swelling, difficulty breathing | Rare | Emergency care first (call 911), then tell your physician |
What is missing matters more than what is reported. The FDA's 2026 review found no clinical safety study, no formal toxicology and no adverse-event reports, which is an absence of data rather than evidence of safety. Anyone with a personal or strong family history of cancer should raise it on the intake, because telomerase activation is the mechanism by which cells escape senescence; see can peptides cause cancer. Epithalon is not used in pregnancy or breastfeeding. It is not named on the 2026 WADA Prohibited List, but the S0 class covers substances without approval for human use, and the ETERNAL Blend contains TB-500 and BPC-157, which are named; tested athletes should check before starting.
Epithalon was removed from Category 2 of the FDA's 503A list on 15 April 2026, and on 24 July 2026 the FDA's Pharmacy Compounding Advisory Committee voted 7 to 4, with one abstention, to recommend adding it to the 503A Bulks List; the FDA's final determination is pending. Compounded medications are not FDA approved.
Epithalon Dosage
Where to get epithalon prescribed
Epithalon cannot be bought legitimately without a prescription. Sites selling it "for research use only" are not accountable for what is in the vial: identity, purity, sterility and concentration are untested, so the concentration a units chart depends on is unknown. The prescription route:
- Complete a free online intake covering your history, medications and what you want help with.
- A physician licensed in your state reviews it, usually within 24 hours.
- If it is appropriate, a PCAB-accredited, FDA-registered US 503A compounding pharmacy prepares it to that prescription. Lot test results are published at /quality/lab-results.
- It ships refrigerated with your directions printed on the vial.
- Your physician stays reachable for questions and dose changes.
Pepti prescribes epithalon in every state except Oklahoma, plus DC Step by step: how to get epithalon prescribed online. Related reading: peptides for sleep and what is Thymalin, its sister peptide from the same institute.
References
What this is based on.
References
- Khavinson VKh, Bondarev IE, Butyugov AA. Epithalon peptide induces telomerase activity and telomere elongation in human somatic cells · Bull Exp Biol Med (2003) · PMID 12937682
- Anisimov VN, Khavinson VKh, Popovich IG et al.. Effect of Epitalon on biomarkers of aging, life span and spontaneous tumor incidence in female Swiss-derived SHR mice · Biogerontology (2003) · PMID 14501183
- Al-Dulaimi S, Thomas R, Matta S, Roberts T. Epitalon increases telomere length in human cell lines through telomerase upregulation or ALT activity · Biogerontology (2025) · PMID 40908429
- Araj SK, Brzezik J, Mądra-Gackowska K, Szeleszczuk Ł. Overview of Epitalon—Highly Bioactive Pineal Tetrapeptide with Promising Properties · Int J Mol Sci (2025) · PMID 40141333
- Khavinson VKh, Morozov VG. Peptides of pineal gland and thymus prolong human life · Neuro Endocrinol Lett (2003) · PMID 14523363
- Korkushko OV, Khavinson VKh, Shatilo VB, Antonyk-Sheglova IA. Peptide geroprotector from the pituitary gland inhibits rapid aging of elderly people: results of 15-year follow-up · Bull Exp Biol Med (2011) · PMID 22451889
- Korkushko OV, Khavinson VKh, Shatilo VB, Magdich LV. Effect of peptide preparation epithalamin on circadian rhythm of epiphyseal melatonin-producing function in elderly people · Bull Exp Biol Med (2004) · PMID 15452611
- Goncharova ND, Khavinson BK, Lapin BA. Regulatory effect of Epithalon on production of melatonin and cortisol in old monkeys · Bull Exp Biol Med (2001) · PMID 11550036
- Djeridane Y, Khavinson VKh, Anisimov VN, Touitou Y. Effect of a synthetic pineal tetrapeptide (Ala-Glu-Asp-Gly) on melatonin secretion by the pineal gland of young and old rats · J Endocrinol Invest (2003) · PMID 12809170
- Khavinson V, Razumovsky M, Trofimova S, Grigorian R, Razumovskaya A. Pineal-regulating tetrapeptide epitalon improves eye retina condition in retinitis pigmentosa · Neuro Endocrinol Lett (2002) · PMID 12195242
- Vinogradova IA, Bukalev AV, Zabezhinski MA, Semenchenko AV et al.. Effect of Ala-Glu-Asp-Gly peptide on life span and development of spontaneous tumors in female rats exposed to different illumination regimes · Bull Exp Biol Med (2007) · PMID 18856211
- Ivko OM, Linkova NS, Ilina AR, Sharova AA, Ryzhak GA. AEDG peptide regulates human circadian rhythms genes expression during pineal gland accelerated aging · Adv Gerontol (2020) · PMID 33280326
Citations are provided for educational purposes. They do not constitute medical advice. Always discuss any peptide protocol with your prescribing physician.
Common questions
Epithalon Dosage, answered.
At Pepti, epithalon is typically prescribed as 0.25 mL, which is 25 units on a U-100 insulin syringe, injected subcutaneously once daily Monday through Friday. From the 2 mg/mL vial that is 0.5 mg per dose. Your physician sets your directions after reviewing your intake, and your exact dose is printed on the vial.
The amount printed on your vial, on the schedule printed on your vial. Pepti's typical directions are 25 units five days a week. If you have questions about your dose, message your care team.
The number printed on your vial. Pepti's typical directions are 25 units, which is 0.25 mL on a U-100 insulin syringe.
From a 2 mg/mL vial, 0.5 mg. 10 units is 0.2 mg and 50 units is 1 mg. Those figures only hold at 2 mg/mL, which is the concentration of both the epithalon vial and the ETERNAL Blend.
The research protocols were intermittent, with courses separated by months, and prescribers generally follow that pattern. One Pepti vial covers about four weeks at the typical directions. How long your course runs is set by your physician after reviewing your intake.
The standard label says once daily without a set time. Because the animal research involved evening hormone levels, it is worth asking your care team; follow any timing printed on your label.
Not for injectable epithalon in people. The FDA found no human pharmacokinetic data and no study of the subcutaneous route in its 2026 review. The human studies quoted online mostly used Epithalamin, a pineal extract, not synthetic epithalon.
About four weeks. A 10 mg vial at 25 units (0.5 mg) five days a week is 20 injections, which uses the whole vial in four weeks, matching the 28-day refill.
Yes for epithalon: 25 units of ETERNAL delivers 0.5 mg, the same as the single vial. Each draw also delivers 2.5 mg of GHK-Cu, 0.5 mg of BPC-157 and 0.5 mg of TB-500.
No. Pepti vials are multi-dose solutions shipped ready to use, with sterile syringes and alcohol prep pads. Nothing is reconstituted at home.
No. Take the dose printed on your vial, on the schedule printed on your vial. The FDA's specific concern about epithalon is long-term, continuous exposure, and taking more or for longer moves in exactly that direction. Message your care team with any questions.
Bloodwork is not required for any Pepti treatment. If your physician wants labs for your care, they'll ask through your account. The free assessment goes to a physician licensed in your state. A consultation does not guarantee a prescription.
Next step
See what a physician recommends for you.
A licensed physician in your state reviews your intake and decides what is appropriate. A consultation does not guarantee a prescription.


