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Anti-Aging · Reference

Epithalon Dosage: How Pepti Prescribes It, in Units, mL and mg

This page explains the epithalon dosage Pepti prescribes, what the units on the syringe mean, a chart that converts units to millilitres and milligrams, the ETERNAL blend that also contains epithalon, how long a vial lasts, why courses are intermittent, what the research did and did not dose, and the side effects to know about. It covers how Pepti's epithalon is typically prescribed, not protocols from the internet. Nothing here is a reason to change your own dose.

Epithalon (Injectable)
Epithalon$249/mo

At Pepti, epithalon is typically prescribed as 0.25 mL, which is 25 units on a U-100 insulin syringe, injected under the skin once daily Monday through Friday. The vial is 5 mL at 2 mg/mL, so each dose carries 0.5 mg of epithalon and the vial holds 20 doses, about four weeks. There is no human dose-finding study for injectable epithalon, so no published human dose exists to compare it with. Your physician sets your directions after reviewing your intake, and your exact dose is printed on the vial.

This page explains the epithalon dosage Pepti prescribes, what the units on the syringe mean, a chart that converts units to millilitres and milligrams, the ETERNAL blend that also contains epithalon, how long a vial lasts, why courses are intermittent, what the research did and did not dose, and the side effects to know about. It covers how Pepti's epithalon is typically prescribed, not protocols from the internet. Nothing here is a reason to change your own dose.

Epithalon Dosage

The short answer

Product Vial Typical directions Epithalon per dose Doses per vial Price
Epithalon 5 mL, 2 mg/mL (10 mg per vial) Inject 0.25 mL (25 units) subcutaneously once daily Monday through Friday 0.5 mg 20 $249/mo
ETERNAL Blend 5 mL, epithalon 2 mg/mL with GHK-Cu, BPC-157 and TB-500 Inject 0.25 mL (25 units) subcutaneously once daily Monday through Friday 0.5 mg, with the other three ingredients 20 $279/mo

Every price is all-in, covering medication, physician review, refill management and shipping, with no separate membership fee. Pepti is cash-pay and does not bill insurance. Epithalon is a vial drawn with an insulin syringe; there is no Pen version. These are the typical printed directions; your own directions are the ones on your vial.

Epithalon Dosage

Epithalon dosage in units, mL and mg

Pepti's directions are written in two forms, millilitres and units, because prescriptions are written in millilitres and insulin syringes are marked in units. On a U-100 insulin syringe, 1 unit is 0.01 mL, so 100 units is 1 mL and 25 units is 0.25 mL. A unit is a volume mark on the barrel, not a measure of how strong the medication is.

The milligram figure comes from the concentration on the label. Pepti's epithalon is supplied at 2 mg/mL, so:

What you read Value Where it comes from
Dose volume 0.25 mL Printed on the vial
On a U-100 insulin syringe 25 units 0.25 mL x 100 units per mL
Concentration 2 mg/mL Printed on the vial
Epithalon per dose 0.5 mg 2 mg/mL x 0.25 mL
Total in the vial 10 mg in 5 mL Printed on the vial
Doses per vial 20 5 mL divided by 0.25 mL

That arithmetic is a way to read a label, not a way to set a dose. A vial labelled 10 mg is not a 10 mg dose: it is twenty 0.5 mg doses. Online epithalon protocols often quote doses for powder vials that the reader mixes at home, at a concentration that depends on how much water was added; a Pepti vial arrives already in solution at a fixed concentration, so those figures do not transfer; do peptides come pre-mixed explains the difference. If your label and your syringe seem to disagree, do not dose; message your care team. How long does a peptide vial last explains every number on a vial label.

Epithalon Dosage

Epithalon dosage chart: units to mL to mg

The chart below is the conversion for a 2 mg/mL vial, drawn with a U-100 insulin syringe. It is math only. It is not a menu of doses, and the only line that applies to you is the one that matches the number printed on your vial.

Syringe mark (U-100) Volume Epithalon from a 2 mg/mL vial
5 units 0.05 mL 0.1 mg
10 units 0.1 mL 0.2 mg
15 units 0.15 mL 0.3 mg
20 units 0.2 mL 0.4 mg
25 units (Pepti's typical directions) 0.25 mL 0.5 mg
30 units 0.3 mL 0.6 mg
40 units 0.4 mL 0.8 mg
50 units 0.5 mL 1 mg
100 units 1 mL 2 mg

The rule behind every row: units divided by 100 gives millilitres, and millilitres multiplied by 2 gives milligrams.

Epithalon in the ETERNAL blend

The ETERNAL Blend holds epithalon at the same 2 mg/mL, so the same 25 unit draw delivers the same 0.5 mg of epithalon. It also delivers the other three ingredients in fixed proportion:

Ingredient Concentration Per 0.25 mL (25 unit) dose Per vial
Epithalon 2 mg/mL 0.5 mg 10 mg
GHK-Cu 10 mg/mL 2.5 mg 50 mg
BPC-157 2 mg/mL 0.5 mg 10 mg
TB-500 2 mg/mL 0.5 mg 10 mg

There is no way to take more epithalon and less of something else from the same vial; a different balance is a different prescription. More on the blend is in what is the ETERNAL blend.

Epithalon Dosage

How long an epithalon vial lasts

At Pepti's typical directions, one 10 mg vial lasts four weeks:

Step Value
Dose 25 units, 0.25 mL, 0.5 mg
Doses per week 5 (Monday through Friday)
Doses per vial 20 (5 mL divided by 0.25 mL)
Epithalon used 20 x 0.5 mg = 10 mg, the whole vial
Time per vial 20 doses divided by 5 per week = 4 weeks

Four weeks is why refills run every 28 days, one vial per fill. Vials are multi-dose solutions shipped ready to use, refrigerated with ice packs, with sterile syringes and alcohol prep pads. Nothing is mixed at home. Keep the vial in the fridge between doses. The FDA's July 2026 briefing noted that reconstituted epithalon is stable for only days at fridge temperature in the manufacturer's data, so the beyond-use date the pharmacy prints on the label governs; it runs from first puncture. How to store peptides covers storage.

If a vial runs short before the refill, message your care team rather than adjusting anything yourself. If you miss a dose, follow your directions and do not take two doses to make up for one.

Epithalon Dosage

When epithalon is taken

  • Once daily, Monday through Friday

    Pepti's directions say once daily Monday through Friday, with weekends off. The weekday-only pattern echoes the intermittent courses used in the Russian research, and it lines a 20-dose vial up with a 28-day refill. Weekends off are part of the schedule, not missed doses.

  • Time of day

    The standard label does not set a time. The animal work involved shifts in evening hormone levels, melatonin and cortisol, so time of day is a reasonable thing to ask your care team about. Follow the timing on your label if one is printed.

  • Under the skin

    Subcutaneous means into the fat layer, not the muscle. Abdomen and thigh are the usual sites, rotated so the same spot is not used repeatedly. There is no reason to inject near any particular body part; the mechanisms described are systemic. Technique is covered in how to inject peptides safely.

    Directions say What it means in practice
    0.25 mL (25 units) Draw to the 25 mark on a U-100 insulin syringe
    Subcutaneously Into the fat layer, abdomen or thigh, rotating sites
    Once daily One injection a day
    Monday through Friday Five doses a week, weekends off
    20 doses per vial About four weeks, then the next refill

Epithalon Dosage

Courses rather than a permanent schedule

The protocols from the St Petersburg institute that developed epithalon were intermittent: courses separated by months. The anchor mouse study, Anisimov and colleagues in Biogerontology in 2003, gave epithalon in brief monthly courses, not continuously. Prescribers generally follow that logic rather than treating epithalon as an indefinite daily medication.

There is also a safety reason. The FDA's July 2026 review raised a concern that continuous, long-term telomerase activation could in principle allow cells to escape senescence, and the rodent work that found fewer tumours used intermittent courses totalling a few months of exposure. How long a course runs, and whether and when it is repeated, is set by your physician after reviewing your intake; do not improvise a permanent schedule. Peptide cycling and tolerance covers how courses work across peptides.

Epithalon Dosage

What the research dosed, and why there is no human reference dose

People search for "the" epithalon dose expecting a trial figure. There is not one to give:

What exists What it was Why it does not give a human dose
Cell studies, 2003 and 2025 Epithalon added to cultured human cells Concentrations in a dish, not doses in a person
Mouse lifespan study, Biogerontology 2003 Brief monthly courses from three months of age, one fixed dose, female mice only An animal dose
Old rhesus monkeys, 2001 A short course that raised evening melatonin An animal dose
Elderly cohorts, 2003 and 2011 Epithalamin, the bovine pineal extract, not synthetic epithalon A different preparation
Night-shift workers, Russian-language study A sublingual spray for 20 days A different route; a urinary melatonin metabolite was measured, not sleep

When the FDA reviewed epithalon in July 2026, it found no human pharmacokinetic data and no study of the subcutaneous route at all. The honest reading is that the injectable dose in common use comes from clinical convention, not a dose-ranging trial. That is why your directions come from a physician who has reviewed your intake. The evidence, one claimed benefit at a time, is in epithalon benefits.

Epithalon Dosage

What changes the directions

Your physician sets your directions after reviewing your intake. Things that can lead to different directions, or a different product, include:

What can change How it shows up
Epithalon alone or in a blend ETERNAL has its own label and its own ingredients
Your medical history A personal or strong family history of cancer, or pregnancy or breastfeeding, change the conversation
Your other medications There is no established interaction list, so the intake asks for everything you take
Course length Courses are intermittent; your physician sets when one ends
How you respond Side effects you report go to your physician, who may revise your directions

When directions change, the new numbers are printed on the new label. Never carry a number over from a previous fill. Bloodwork is not required for any Pepti treatment. If your physician wants labs for your care, they'll ask through your account.

Epithalon Dosage

Side effects, in summary

The full picture is in what is epithalon.

What is reported What it usually means What to do
Injection-site redness, mild soreness, occasional bruising The commonest complaint Rotate sites
Early drowsiness or vivid dreams Usually settles Report it if it persists
A severe or spreading reaction, facial or throat swelling, difficulty breathing Rare Emergency care first (call 911), then tell your physician

What is missing matters more than what is reported. The FDA's 2026 review found no clinical safety study, no formal toxicology and no adverse-event reports, which is an absence of data rather than evidence of safety. Anyone with a personal or strong family history of cancer should raise it on the intake, because telomerase activation is the mechanism by which cells escape senescence; see can peptides cause cancer. Epithalon is not used in pregnancy or breastfeeding. It is not named on the 2026 WADA Prohibited List, but the S0 class covers substances without approval for human use, and the ETERNAL Blend contains TB-500 and BPC-157, which are named; tested athletes should check before starting.

Epithalon was removed from Category 2 of the FDA's 503A list on 15 April 2026, and on 24 July 2026 the FDA's Pharmacy Compounding Advisory Committee voted 7 to 4, with one abstention, to recommend adding it to the 503A Bulks List; the FDA's final determination is pending. Compounded medications are not FDA approved.

Epithalon Dosage

Where to get epithalon prescribed

Epithalon cannot be bought legitimately without a prescription. Sites selling it "for research use only" are not accountable for what is in the vial: identity, purity, sterility and concentration are untested, so the concentration a units chart depends on is unknown. The prescription route:

  1. Complete a free online intake covering your history, medications and what you want help with.
  2. A physician licensed in your state reviews it, usually within 24 hours.
  3. If it is appropriate, a PCAB-accredited, FDA-registered US 503A compounding pharmacy prepares it to that prescription. Lot test results are published at /quality/lab-results.
  4. It ships refrigerated with your directions printed on the vial.
  5. Your physician stays reachable for questions and dose changes.

Pepti prescribes epithalon in every state except Oklahoma, plus DC Step by step: how to get epithalon prescribed online. Related reading: peptides for sleep and what is Thymalin, its sister peptide from the same institute.

References

What this is based on.

References

  1. Khavinson VKh, Bondarev IE, Butyugov AA. Epithalon peptide induces telomerase activity and telomere elongation in human somatic cells · Bull Exp Biol Med (2003) · PMID 12937682
  2. Anisimov VN, Khavinson VKh, Popovich IG et al.. Effect of Epitalon on biomarkers of aging, life span and spontaneous tumor incidence in female Swiss-derived SHR mice · Biogerontology (2003) · PMID 14501183
  3. Al-Dulaimi S, Thomas R, Matta S, Roberts T. Epitalon increases telomere length in human cell lines through telomerase upregulation or ALT activity · Biogerontology (2025) · PMID 40908429
  4. Araj SK, Brzezik J, Mądra-Gackowska K, Szeleszczuk Ł. Overview of Epitalon—Highly Bioactive Pineal Tetrapeptide with Promising Properties · Int J Mol Sci (2025) · PMID 40141333
  5. Khavinson VKh, Morozov VG. Peptides of pineal gland and thymus prolong human life · Neuro Endocrinol Lett (2003) · PMID 14523363
  6. Korkushko OV, Khavinson VKh, Shatilo VB, Antonyk-Sheglova IA. Peptide geroprotector from the pituitary gland inhibits rapid aging of elderly people: results of 15-year follow-up · Bull Exp Biol Med (2011) · PMID 22451889
  7. Korkushko OV, Khavinson VKh, Shatilo VB, Magdich LV. Effect of peptide preparation epithalamin on circadian rhythm of epiphyseal melatonin-producing function in elderly people · Bull Exp Biol Med (2004) · PMID 15452611
  8. Goncharova ND, Khavinson BK, Lapin BA. Regulatory effect of Epithalon on production of melatonin and cortisol in old monkeys · Bull Exp Biol Med (2001) · PMID 11550036
  9. Djeridane Y, Khavinson VKh, Anisimov VN, Touitou Y. Effect of a synthetic pineal tetrapeptide (Ala-Glu-Asp-Gly) on melatonin secretion by the pineal gland of young and old rats · J Endocrinol Invest (2003) · PMID 12809170
  10. Khavinson V, Razumovsky M, Trofimova S, Grigorian R, Razumovskaya A. Pineal-regulating tetrapeptide epitalon improves eye retina condition in retinitis pigmentosa · Neuro Endocrinol Lett (2002) · PMID 12195242
  11. Vinogradova IA, Bukalev AV, Zabezhinski MA, Semenchenko AV et al.. Effect of Ala-Glu-Asp-Gly peptide on life span and development of spontaneous tumors in female rats exposed to different illumination regimes · Bull Exp Biol Med (2007) · PMID 18856211
  12. Ivko OM, Linkova NS, Ilina AR, Sharova AA, Ryzhak GA. AEDG peptide regulates human circadian rhythms genes expression during pineal gland accelerated aging · Adv Gerontol (2020) · PMID 33280326

Citations are provided for educational purposes. They do not constitute medical advice. Always discuss any peptide protocol with your prescribing physician.

Common questions

Epithalon Dosage, answered.

At Pepti, epithalon is typically prescribed as 0.25 mL, which is 25 units on a U-100 insulin syringe, injected subcutaneously once daily Monday through Friday. From the 2 mg/mL vial that is 0.5 mg per dose. Your physician sets your directions after reviewing your intake, and your exact dose is printed on the vial.

Next step

See what a physician recommends for you.

A licensed physician in your state reviews your intake and decides what is appropriate. A consultation does not guarantee a prescription.