Anti-Aging · Reference
FOXO4-DRI Dosage: How Pepti Prescribes It, in Units, mL and mg
This page explains the FOXO4-DRI dosage Pepti prescribes, what the units on the syringe mean, a conversion chart for the vial, why the weekday schedule exists, why the published research cannot supply a human dose, and what can change your directions. It covers how Pepti prescribes FOXO4-DRI, not online protocols. Nothing here is a reason to change your own dose.
Treatments mentioned
At Pepti, FOXO4-DRI is prescribed as 0.25 mL, which is 25 units on a U-100 insulin syringe, injected under the skin once daily Monday through Friday. The 5 mL vial holds 10 mg at 2 mg/mL, so every 0.25 mL dose delivers 0.5 mg of FOXO4-DRI, and the vial holds 20 doses, about four weeks. There is no human dose study of FOXO4-DRI of any kind, so this schedule is a prescribing standard, not a trial result. Your physician sets your directions after reviewing your intake, and your exact dose and units are printed on the vial.
This page explains the FOXO4-DRI dosage Pepti prescribes, what the units on the syringe mean, a conversion chart for the vial, why the weekday schedule exists, why the published research cannot supply a human dose, and what can change your directions. It covers how Pepti prescribes FOXO4-DRI, not online protocols. Nothing here is a reason to change your own dose.
FOXO4-DRI Dosage
The short answer
| Product | Vial | Directions | Per dose | Doses per vial | Price |
|---|---|---|---|---|---|
| FOXO4-DRI | 5 mL multi-dose vial, 2 mg/mL (10 mg per vial) | Inject 0.25 mL (25 units) subcutaneously once daily Monday through Friday | 0.5 mg | 20 | $229/mo |
The price is all-in, covering medication, physician review, refill management and shipping, with no separate membership fee. Pepti is cash-pay and does not bill insurance. Your own directions are the ones printed on your vial.
FOXO4-DRI Dosage
FOXO4-DRI dosage in units, mL and mg
Pepti's directions are written in millilitres and units, because prescriptions are written in millilitres and insulin syringes are marked in units. On a U-100 insulin syringe, 1 unit is 0.01 mL, so 100 units is 1 mL and 25 units is 0.25 mL. A unit is a volume mark on the barrel, not a measure of how strong the medication is.
The milligrams come from the vial's concentration. FOXO4-DRI is supplied at 2 mg/mL, so:
| Step | Value |
|---|---|
| Concentration | 2 mg/mL (10 mg in 5 mL) |
| Printed volume | 0.25 mL |
| Syringe mark | 25 units on a U-100 insulin syringe |
| Arithmetic | 0.25 mL multiplied by 2 mg/mL |
| FOXO4-DRI per dose | 0.5 mg (500 micrograms) |
That arithmetic is a way to read a label, not a way to set a dose. A vial labelled 10 mg is not a 10 mg dose: it is twenty doses of 0.5 mg. If your label and your syringe seem to disagree, do not dose; message your care team. How long does a peptide vial last explains every number on a vial label.
FOXO4-DRI Dosage
FOXO4-DRI dosage chart: units to mL to mg
The chart below is the conversion for a 2 mg/mL FOXO4-DRI vial, drawn with a U-100 insulin syringe. It is math only. It is not a menu of doses, and the only line that applies to you is the one that matches the number printed on your vial.
| Syringe mark (U-100) | Volume | FOXO4-DRI |
|---|---|---|
| 5 units | 0.05 mL | 0.1 mg |
| 10 units | 0.1 mL | 0.2 mg |
| 15 units | 0.15 mL | 0.3 mg |
| 20 units | 0.2 mL | 0.4 mg |
| 25 units (Pepti's typical directions) | 0.25 mL | 0.5 mg |
| 50 units | 0.5 mL | 1 mg |
| 100 units | 1 mL | 2 mg |
The rule behind every row: units divided by 100 gives millilitres, and millilitres multiplied by 2 gives milligrams. That multiplier only holds for a 2 mg/mL vial. A chart written for a different concentration gives different milligram figures for the same units, which is the main reason not to dose from a chart, including this one.
FOXO4-DRI Dosage
Why the research cannot give you a FOXO4-DRI dose
This is the most important section on the page, because FOXO4-DRI is different from most peptides people look up.
| What a dose normally rests on | What exists for FOXO4-DRI |
|---|---|
| A human dose-finding study | None |
| Human pharmacokinetics (how long it lasts, how it is cleared) | None |
| A human safety study | None |
| Randomised human trials | None, and no non-randomised human trials either |
| Animal dosing | Short courses of intermittent intraperitoneal injection in mice (Cell, 2017) |
The mouse work that made the compound known used a short, intermittent course injected into the abdominal cavity, which is not a route used in people for this purpose. Effects were measured over days to a few weeks, in an animal that ages in two years. There is no established way to convert a mouse dose or schedule to a human one, and online "protocols" that quote a human dose from those papers are doing a conversion nobody has validated.
So the directions on the vial are a clinical convention: prescribers treat FOXO4-DRI as an intermittent course rather than a continuous daily medication, which loosely mirrors the intermittent dosing in the mouse work, and the vial is sized to last about four weeks. The subcutaneous route is a clinical choice, not a translated one. What the research does describe is set out in FOXO4-DRI benefits and what is FOXO4-DRI.
FOXO4-DRI Dosage
When FOXO4-DRI is taken
Once daily, Monday through Friday
Pepti's FOXO4-DRI directions say once daily, Monday through Friday, with weekends off. The weekend break is part of the schedule, not two missed doses. Twenty doses at five a week is four working weeks, which is why refills run on a 28-day cycle. The directions do not name a time of day; if yours do, follow the label.
Under the skin, and where
Subcutaneous means into the fat layer, not the muscle. Abdomen and thigh are the usual sites, rotated so the same spot is not used repeatedly. Technique is covered in how to inject peptides safely.
| Directions say | What it means in practice |
|---|---|
| 0.25 mL (25 units) | Draw to the 25 mark on a U-100 insulin syringe |
| Subcutaneously | Into the fat layer, abdomen or thigh, rotating sites |
| Once daily | One injection on each dosing day |
| Monday through Friday | Five doses a week, weekends off |
| 20 doses per vial | About four weeks |
FOXO4-DRI Dosage
How long a FOXO4-DRI vial lasts, storage and refills
| Step | Value |
|---|---|
| Dose | 25 units, 0.25 mL, 0.5 mg |
| Doses per week | 5 (Monday through Friday) |
| Doses per vial | 20 (5 mL divided by 0.25 mL) |
| Used over the vial | 10 mg, the whole vial |
| Time per vial | 20 doses divided by 5 per week = 4 weeks |
Vials are multi-dose solutions shipped ready to use, refrigerated with ice packs, with sterile syringes and alcohol prep pads. Nothing is mixed at home. The D-amino acid chemistry makes the peptide more stable than a natural sequence, but the compounded solution is still handled as a refrigerated multi-dose vial; follow the beyond-use date on the label. Cloudiness, particles or a change from what you received is a reason to message rather than inject. One vial per fill. How to store peptides covers storage.
If a vial runs short, message your care team rather than adjusting anything yourself. If you miss a weekday dose, follow your directions and do not take two doses to make up for one.
Is FOXO4-DRI taken continuously?
Prescribers generally treat it as a course. Whether a second vial is prescribed is reviewed with each refill, and with an experimental compound it is reasonable for that answer to change, including to no. There is no withdrawal and no rebound described in the animal work, and whether senescent cells re-accumulate after a course, and how fast, is unknown in humans.
FOXO4-DRI Dosage
FOXO4-DRI next to the other anti-aging products
People sometimes compare a FOXO4-DRI dose with other anti-aging vials. The draw looks the same, but the products are not interchangeable:
| FOXO4-DRI | Epithalon | MOTS-c | SS-31 | |
|---|---|---|---|---|
| Vial | 10 mg in 5 mL (2 mg/mL) | 10 mg in 5 mL (2 mg/mL) | 10 mg in 5 mL (2 mg/mL) | 10 mg in 5 mL (2 mg/mL) |
| What it is aimed at | Removing senescent cells | Telomere and pineal biology | Mitochondrial metabolic signalling | The inner mitochondrial membrane |
| Human data | None | Older Russian work | Observational only | Randomised trials of elamipretide in rare mitochondrial disease and heart failure |
| Price | $229/mo | $249/mo | $259/mo | $249/mo |
Same concentration does not mean same dose in any meaningful sense, because each milligram is a different molecule with a different target. Each product's directions are its own, printed on its own vial. The comparisons are covered in peptides for longevity and anti-aging.
FOXO4-DRI Dosage
What changes the directions
Your physician sets your directions after reviewing your intake. Things that can lead to different directions, or a different product, include:
| What can change | How it shows up |
|---|---|
| Whether FOXO4-DRI is the right product | A wish for an intervention with human data may point to NAD+ or tesamorelin; energy and metabolism to MOTS-c or SS-31 |
| A history of cancer | FOXO4-DRI acts on p53, the central tumour-suppressor protein |
| Lung disease or pulmonary hypertension | A 2023 mouse study in Circulation found senolytics worsened pulmonary hypertension |
| Pregnancy or breastfeeding | Not used; safety data is absent |
| Whether a further course is appropriate | Reviewed with each refill |
| How you respond | Reactions you report go to your physician, who may revise your directions |
When directions change, the new numbers are printed on the new label. Never carry a number over from a previous fill. Bloodwork is not required for any Pepti treatment. If your physician wants labs for your care, they'll ask through your account.
FOXO4-DRI Dosage
Side effects, in summary
| What is known | What to do |
|---|---|
| Injection-site redness, mild soreness, occasional bruising | Rotate sites |
| Well tolerated in mice at the doses used; no human safety data | Report anything new or persistent |
| Immune reactions to unusual peptide chemistry are a theoretical concern | Report a rash, hives or a reaction away from the site |
| Facial or throat swelling, widespread rash, breathing difficulty | Call 911 first, then tell your physician |
If you think you may have taken too much, call Poison Control at 1-800-222-1222 and message your care team. Do not skip, double or change a dose to compensate. FOXO4-DRI is a compounded prescription; compounded medications are not FDA approved, and the FDA has not evaluated FOXO4-DRI in any category. Tested athletes should check the current WADA list with their anti-doping body before starting any peptide.
FOXO4-DRI Dosage
Where to get FOXO4-DRI prescribed
FOXO4-DRI cannot be bought legitimately without a prescription. Sites selling it "for research use only" are not accountable for what is in the vial: identity, purity, sterility and concentration are untested, so the per-dose figures above would not hold. See research peptides vs prescription peptides. The prescription route:
- Complete a free online intake covering your history, medications and what you want help with.
- A physician licensed in your state reviews it, usually within 24 hours.
- If it is appropriate, a PCAB-accredited, FDA-registered US 503A compounding pharmacy prepares it to that prescription. Lot test results are published at /quality/lab-results.
- It ships refrigerated with your directions printed on the vial.
- Your physician stays reachable for questions and dose changes.
Pepti prescribes FOXO4-DRI in every state except Oklahoma, plus DC
References
What this is based on.
References
- Baar MP, Brandt RMC, Putavet DA et al.. Targeted Apoptosis of Senescent Cells Restores Tissue Homeostasis in Response to Chemotoxicity and Aging · Cell (2017) · PMID 28340339
- Bourgeois B, Spreitzer E, Platero-Rochart D et al.. The disordered p53 transactivation domain is the target of FOXO4 and the senolytic compound FOXO4-DRI · Nature Communications (2025) · PMID 40593617
- Zhang C, Xie Y, Chen H et al.. FOXO4-DRI alleviates age-related testosterone secretion insufficiency by targeting senescent Leydig cells in aged mice · Aging (Albany NY) (2020) · PMID 31959736
- Huang Y, He Y, Makarcyzk MJ et al.. Senolytic Peptide FOXO4-DRI Selectively Removes Senescent Cells From in vitro Expanded Human Chondrocytes · Frontiers in Bioengineering and Biotechnology (2021) · PMID 33996787
- Liu Y, Hou Q, Wang R et al.. FOXO4-D-Retro-Inverso targets extracellular matrix production in fibroblasts and ameliorates bleomycin-induced pulmonary fibrosis in mice · Naunyn-Schmiedeberg's Archives of Pharmacology (2023) · PMID 37074394
- Li Y, Zhang C, Cheng H et al.. FOXO4-DRI improves spermatogenesis in aged mice through reducing senescence-associated secretory phenotype secretion from Leydig cells · Experimental Gerontology (2024) · PMID 39025385
- Hu Z, Li F, Hu C et al.. FOXO4-DRI regulates endothelial cell senescence via the P53 signaling pathway · Frontiers in Bioengineering and Biotechnology (2026) · PMID 41625068
- Kong YX, Li ZS, Liu YB et al.. FOXO4-DRI induces keloid senescent fibroblast apoptosis by promoting nuclear exclusion of upregulated p53-serine 15 phosphorylation · Communications Biology (2025) · PMID 39994346
- Born E, Lipskaia L, Breau M et al.. Eliminating Senescent Cells Can Promote Pulmonary Hypertension Development and Progression · Circulation (2023) · PMID 36515093
Citations are provided for educational purposes. They do not constitute medical advice. Always discuss any peptide protocol with your prescribing physician.
Common questions
FOXO4-DRI Dosage, answered.
At Pepti, 0.25 mL, which is 25 units on a U-100 insulin syringe, injected subcutaneously once daily Monday through Friday. At 2 mg/mL that is 0.5 mg per dose. Your physician sets your directions after reviewing your intake, and your exact dose is printed on the vial.
At Pepti's typical directions, 0.5 mg (500 micrograms): 0.25 mL of a 2 mg/mL solution.
The number printed on your vial. Pepti's typical directions are 25 units, which is 0.25 mL on a U-100 insulin syringe, where 1 unit is 0.01 mL.
Twenty. A 5 mL vial divided by 0.25 mL per dose is 20 doses, four working weeks on a Monday to Friday schedule.
The chart above converts units to millilitres to milligrams for a 2 mg/mL vial. It is a conversion, not a list of doses to choose from. Your own label is the one that applies.
The published studies are in mice, using short courses of intermittent intraperitoneal injection. There is no established way to convert those to a human dose, and no human dose study exists.
Because prescribers treat it as an intermittent course rather than a continuous daily medication, loosely mirroring the intermittent dosing in the mouse work. The Monday-through-Friday schedule is a clinical convention, not a trial-established regimen.
At Pepti there is one set of directions on the label, set by your physician after reviewing your intake. There is no separate starter protocol to work out yourself.
One vial lasts about four weeks. Whether a further vial is prescribed is reviewed with each refill, and with an experimental compound the answer can reasonably be no.
No. Take the dose printed on your vial, on the schedule printed on your vial. If you have questions about your dose, message your care team.
No. It is supplied as a vial with sterile syringes. Vial vs pen explains how the formats differ.
No. Pepti vials are multi-dose solutions shipped ready to use, with sterile syringes and alcohol prep pads.
Bloodwork is not required for any Pepti treatment. If your physician wants labs for your care, they'll ask through your account. The free assessment goes to a physician licensed in your state. A consultation does not guarantee a prescription.
Next step
See what a physician recommends for you.
A licensed physician in your state reviews your intake and decides what is appropriate. A consultation does not guarantee a prescription.