Supplements · Reference
B12 Dosage: How Pepti Prescribes B12 Injections, in Units, mL and mg
This page explains the B12 dosage Pepti prescribes, what the units mean, conversion charts for each vial, when the doses are taken, how a vial maps onto refills, what can change your directions, and the side effects to know about. It covers how Pepti prescribes B12, not online protocols or deficiency treatment schedules. Nothing here is a reason to change your own dose.

Treatments mentioned
At Pepti, methylcobalamin (active B12) is prescribed as 1.25 mL, which is 125 units on a U-100 syringe scale, injected into the muscle once weekly. The vial is 5 mL at 2 mg/mL, so each dose carries 2.5 mg of methylcobalamin and the vial holds 4 doses, about four weeks. Lipo-B, which is B12 plus methionine, inositol and choline, is prescribed as 0.8 mL (80 units) into the muscle three times weekly, which is 0.8 mg of B12 per dose and 12 doses per 10 mL vial, also about four weeks. Your physician sets your directions after reviewing your intake, and your exact dose is printed on the vial.
This page explains the B12 dosage Pepti prescribes, what the units mean, conversion charts for each vial, when the doses are taken, how a vial maps onto refills, what can change your directions, and the side effects to know about. It covers how Pepti prescribes B12, not online protocols or deficiency treatment schedules. Nothing here is a reason to change your own dose.
B12 Dosage
The short answer
| Product | Vial | Directions | B12 per dose | Doses per vial | Price |
|---|---|---|---|---|---|
| Methylcobalamin (Active B12) | 5 mL, 2 mg/mL (10 mg per vial) | Inject 1.25 mL (125 units) intramuscularly once weekly | 2.5 mg methylcobalamin | 4 | $229/mo |
| Lipo-B (B12 + MIC) | 10 mL; per mL 50 mg methionine, 50 mg inositol, 25 mg choline, 1 mg B12 | Inject 0.8 mL (80 units) intramuscularly three times weekly | 0.8 mg cyanocobalamin | 12 | $209/mo |
| Lipo-Mino-Mix-C | 10 mL, MIC with vitamins B6, B1 and B12 | Inject 0.8 mL (80 units) intramuscularly three times weekly | Printed on the label | 12 | $209/mo |
Every price is all-in, covering medication, physician review, refill management and shipping, with no separate membership fee. Pepti is cash-pay and does not bill insurance. Your own directions are the ones printed on your vial.
B12 Dosage
Methylcobalamin dosage in units, mL and mg
Pepti's directions are written two ways, millilitres and units, because prescriptions are written in millilitres and syringes are marked in units. On a U-100 syringe, 1 unit is 0.01 mL, so 100 units is 1 mL and 125 units is 1.25 mL. A unit is a volume mark on the barrel, not a measure of strength.
The milligram figure comes from the concentration on the label. Methylcobalamin is supplied at 2 mg/mL, so:
| What you read | Value | Where it comes from |
|---|---|---|
| Dose volume | 1.25 mL | Printed on the vial |
| In units | 125 units | 1.25 mL x 100 units per mL |
| Concentration | 2 mg/mL | Printed on the vial |
| Methylcobalamin per dose | 2.5 mg | 2 mg/mL x 1.25 mL |
| Total in the vial | 10 mg in 5 mL | Printed on the vial |
| Doses per vial | 4 | 5 mL divided by 1.25 mL |
| How long the vial lasts | About four weeks | 4 doses at once weekly |
Methylcobalamin conversion chart (2 mg/mL vial)
This is arithmetic for reading a 2 mg/mL label, not a dosing guide. Your dose is the one printed on your vial.
| Units (U-100) | mL | Methylcobalamin |
|---|---|---|
| 10 units | 0.10 mL | 0.2 mg |
| 25 units | 0.25 mL | 0.5 mg |
| 50 units | 0.50 mL | 1.0 mg |
| 75 units | 0.75 mL | 1.5 mg |
| 100 units | 1.00 mL | 2.0 mg |
| 125 units | 1.25 mL | 2.5 mg |
A vial labelled 10 mg is not a 10 mg dose: it is four 2.5 mg doses. If your label and your syringe seem to disagree, do not inject; message your care team. How long does a peptide vial last explains every number on a vial label.
B12 Dosage
Lipo-B dosage in units, mL and mg
Lipo-B's label reads "50mg/50mg/25mg/1mg/mL." Those four numbers are the concentration of each ingredient per millilitre, in order: methionine, inositol, choline and vitamin B12 (cyanocobalamin). The vial holds 10 mL.
| What you read | Value | Where it comes from |
|---|---|---|
| Dose volume | 0.8 mL | Printed on the vial |
| In units | 80 units | 0.8 mL x 100 units per mL |
| Methionine per dose | 40 mg | 50 mg/mL x 0.8 mL |
| Inositol per dose | 40 mg | 50 mg/mL x 0.8 mL |
| Choline per dose | 20 mg | 25 mg/mL x 0.8 mL |
| B12 per dose | 0.8 mg | 1 mg/mL x 0.8 mL |
| Doses per vial | 12 | 12 x 0.8 mL is 9.6 mL of the 10 mL vial, leaving a little for draw losses |
| How long the vial lasts | About four weeks | 12 doses at three times weekly |
Lipo-B conversion chart (50/50/25/1 mg per mL)
Again, label arithmetic only, not a dosing guide.
| Units (U-100) | mL | Methionine | Inositol | Choline | B12 |
|---|---|---|---|---|---|
| 20 units | 0.2 mL | 10 mg | 10 mg | 5 mg | 0.2 mg |
| 40 units | 0.4 mL | 20 mg | 20 mg | 10 mg | 0.4 mg |
| 50 units | 0.5 mL | 25 mg | 25 mg | 12.5 mg | 0.5 mg |
| 80 units | 0.8 mL | 40 mg | 40 mg | 20 mg | 0.8 mg |
| 100 units | 1.0 mL | 50 mg | 50 mg | 25 mg | 1.0 mg |
In Lipo-B every ingredient is in fixed proportion. One draw delivers all four in the ratio the pharmacy compounded; there is no way to take more B12 and less of something else from the same vial. A different balance means a different prescription.
Lipo-Mino-Mix-C
Lipo-Mino-Mix-C is drawn the same way, 0.8 mL (80 units) intramuscularly three times weekly, 12 doses per 10 mL vial. It contains methionine, inositol and choline with vitamins B6, B1 and B12; its per-millilitre amounts are on its label and in what is Lipo-Mino-Mix-C.
B12 Dosage
Methylcobalamin vs Lipo-B, dose for dose
| Methylcobalamin | Lipo-B | |
|---|---|---|
| Form of B12 | Methylcobalamin | Cyanocobalamin |
| Volume per dose | 1.25 mL (125 units) | 0.8 mL (80 units) |
| Doses per week | 1 | 3 |
| B12 per dose | 2.5 mg | 0.8 mg |
| B12 per week | 2.5 mg | 2.4 mg |
| Other ingredients | None | 40 mg methionine, 40 mg inositol, 20 mg choline per dose |
| Doses per vial | 4 | 12 |
| Route | Intramuscular | Intramuscular |
The weekly B12 totals land close together, by different schedules. The forms differ, but for treating or preventing deficiency the review literature finds them broadly interchangeable, because cells strip the upper group off every form of B12 and rebuild the working coenzymes themselves. Which product fits is about what else is in the vial and why it is being prescribed; B12 injections covers that choice.
B12 Dosage
What published studies used
The research on B12 is mostly about correcting deficiency, and the sources Pepti relies on report few specific injected doses. What they do report:
| Source | What it used | What it tells you |
|---|---|---|
| Blood, 1998 | 2 mg oral B12 daily versus a standard intramuscular schedule, in 38 newly deficient patients | High-dose oral B12 corrected deficiency about as well as injections in that trial |
| Cochrane review, updated 2018 | Three trials, 153 participants, oral versus intramuscular | Similar effects on serum levels, on low-quality evidence |
| Oral myo-inositol trials in PCOS (2023 meta-analysis) | 2 to 4 grams of inositol a day by mouth | Roughly 50 to 100 times the 40 mg in a Lipo-B dose, so not transferable |
| US Institute of Medicine | Choline adequate intake of 550 mg a day for men, 425 mg for women | A Lipo-B dose holds 20 mg |
None of these is a reason to change a printed dose. Deficiency treatment in medicine generally may use different schedules, set from a person's levels; your directions are set for you.
B12 Dosage
When B12 is taken
Once weekly, or three times weekly
Methylcobalamin is directed once weekly, which makes the vial arithmetic clean: four doses, four weeks. Lipo-B and Lipo-Mino-Mix-C are directed three times weekly, with the spacing in your directions, and 12 doses at three a week is also four weeks. There is no stated time of day in Pepti's B12 directions.
Into the muscle
Intramuscular means into the muscle, not the fat layer under the skin. That is the traditional route for B12, and the one Pepti's directions specify for all three products. The usual sites are the outer thigh and the upper outer buttock, rotated between doses. The pharmacy supplies syringes and needles suited to the route, with alcohol prep pads. Do not switch to injecting under the skin on your own; ask your care team. General technique is in how to inject peptides safely.
| Directions say | What it means in practice |
|---|---|
| 1.25 mL (125 units) | Methylcobalamin: draw to the 125-unit volume, 1.25 mL |
| 0.8 mL (80 units) | Lipo-B or Lipo-Mino-Mix-C: draw to 80 units |
| Intramuscularly | Into the muscle, outer thigh or upper outer buttock, rotating sites |
| Once weekly | One dose a week |
| Three times weekly | Three doses a week, spaced as your directions say |
B12 Dosage
How a vial maps onto refills
Each vial is sized to last about four weeks at the printed directions, which is why refills run every 28 days, one vial per fill. Vials are multi-dose solutions shipped ready to use, refrigerated with ice packs, with sterile syringes and alcohol prep pads. Nothing is mixed at home. B12 is light-sensitive, so keep the vial in its carton and out of direct light, at the storage temperature printed on the label. The beyond-use date is on the label and runs from first puncture; a vial that has changed color or gone cloudy should not be used. How to store peptides covers storage.
If a vial runs short before the refill, message your care team rather than adjusting anything yourself. If you miss a dose, follow your directions and do not take two doses to make up for one.
B12 Dosage
What changes the directions
Your physician sets your directions after reviewing your intake. Things that can lead to different directions, or a different product, include:
| What can change | How it shows up |
|---|---|
| Which product is prescribed | Methylcobalamin alone, Lipo-B or Lipo-Mino-Mix-C; each has its own label |
| Why B12 is being prescribed | Maintaining B12 status is a different conversation from support alongside a weight plan |
| Your medical history | Pernicious anemia, stomach or bowel surgery, a strictly plant-based diet or long-term metformin are ongoing reasons for B12 |
| Your other medications | Metformin, acid suppressants, colchicine and high-dose folic acid all belong on the intake |
| How you respond | Side effects you report go to your physician, who may revise your directions |
When directions change, the new numbers are printed on the new label. Never carry a number over from a previous fill. Bloodwork is not required for any Pepti treatment. If your physician wants labs for your care, they'll ask through your account.
B12 Dosage
Side effects, in summary
The full list is in B12 side effects.
| What is reported | What it usually means | What to do |
|---|---|---|
| Stinging, soreness or bruising at the site | The commonest complaint; intramuscular shots can sting more | Rotate sites |
| Brief warmth or flushing (Lipo-B) | Reported with the injection | Mention it to your care team |
| Acne-like spots, mild diarrhea, headache | Less common | Message your care team if it persists |
| Facial or throat swelling, spreading rash, trouble breathing | Rare allergic reaction | Call 911, then tell your physician |
B12 has no established upper intake level; excess is excreted in urine. Even so, inject only what is printed on your vial. If you think you have injected far more than directed, call Poison Control at 1-800-222-1222 and message your care team.
Cyanocobalamin injection is an FDA-approved drug. Methylcobalamin is in 503A Category 1, under FDA evaluation, and Lipo-B is a compounded combination. Compounded medications are not FDA approved.
B12 Dosage
Where to get B12 injections prescribed
Prescription B12 and MIC injections should come from a licensed pharmacy filling a prescription written for you. The prescription route:
- Complete a free online intake covering your history, medications and what you want help with.
- A physician licensed in your state reviews it, usually within 24 hours.
- If it is appropriate, a PCAB-accredited, FDA-registered US 503A compounding pharmacy prepares it to that prescription. Lot test results are published at /quality/lab-results.
- It ships refrigerated with your directions printed on the vial.
- Your physician stays reachable for questions and dose changes.
Pepti prescribes Methylcobalamin and Lipo-B in every state except Oklahoma, plus DC The full product references are what is methylcobalamin and what is Lipo-B; what the research describes is in B12 benefits.
Common questions
B12 Dosage, answered.
At Pepti, methylcobalamin is 1.25 mL (125 units) intramuscularly once weekly, 2.5 mg per dose. Lipo-B is 0.8 mL (80 units) intramuscularly three times weekly, 0.8 mg of B12 per dose. Your physician sets your directions after reviewing your intake, and your exact dose is printed on the vial.
The number printed on your vial. Pepti's standard directions are 125 units (1.25 mL) for methylcobalamin and 80 units (0.8 mL) for Lipo-B. On a U-100 scale, 1 unit is 0.01 mL.
It depends on the vial. Pepti's methylcobalamin is 2 mg per mL. Lipo-B holds 1 mg of B12 per mL, alongside 50 mg methionine, 50 mg inositol and 25 mg choline. Read your own label.
Under Pepti's directions, methylcobalamin is once weekly and Lipo-B is three times weekly. Both vials last about four weeks, matching a 28-day refill.
Four in the 5 mL methylcobalamin vial at 1.25 mL each, and twelve in the 10 mL Lipo-B vial at 0.8 mL each.
B12 has no established upper intake level, and what the body does not use is excreted in urine. The 1998 Blood trial used 2 mg of oral B12 daily. Your dose is set for you and printed on the vial; do not change it on your own.
0.8 mL (80 units) intramuscularly three times weekly. Each dose holds 40 mg methionine, 40 mg inositol, 20 mg choline and 0.8 mg B12.
Pepti's B12 directions are intramuscular: the outer thigh or the upper outer buttock, rotating sites. Do not switch to injecting under the skin on your own; ask your care team.
Follow the directions on your vial and do not take two doses to make up for one. If you are unsure, message your care team.
No. Pepti vials are multi-dose solutions shipped ready to use, with sterile syringes and alcohol prep pads. Nothing is mixed at home.
Bloodwork is not required for any Pepti treatment. If your physician wants labs for your care, they'll ask through your account. The free assessment goes to a physician licensed in your state. A consultation does not guarantee a prescription.
Next step
See what a physician recommends for you.
A licensed physician in your state reviews your intake and decides what is appropriate. A consultation does not guarantee a prescription.

